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What's "normal", anyway? Marvin was 13 when he went missing after being taken into a nearby care home after the death of his father.
He said goodbye to his carers before leaving to meet friends on June 11, in the town centre. He sent a final WhatsApp message at Police had been prepared to give up.
His discovery seems to have been by accident. At 7am on Friday police visited the home of Lars H with a van and a dog trained to sniff out hidden computer hard drives - suspected of containing child abuse images.
They found binbags full of used nappies - and an overwhelming smell of urine. The smell was so severe investigators had to work with face masks on.
And when officers opened a cupboard door an uninjured Marvin was standing in the darkness. He's exhausted, too. She added: 'He said, 'Mummy, I was locked away for two and a half years and I couldn't get any fresh air'.
He said, 'Mummy, take me home. He was actually wearing the same clothes he had on the day he disappeared. Lars H and a year-old man - reported to be his father - were arrested.
The older man was released but Lars H, an unemployed handyman, was kept in custody over the weekend.
It is unclear whether he will be charged. Schools are allowed to have December 18 off so 'stressed' teachers get a 'proper break' - after The authors did not find elevated numbers of induced micronuclei in Hep G2 cells after arsenic exposure.
A cell-cycle delay in arsenite-treated human leukaemia cells was reported by McCabe et al. Kligerman et al.
In our study, a recovery period was instituted, i. Hence, in contrast to the results of McCabe et al. Taken together, these data indicate that arsenic compounds are able to temporarily delay the cell cycle, but that this delay is reversible.
These results are in agreement with data of previous studies published by Dopp et al. After an extended exposure time of 24 h and a recovery period of 14 h in the present study, the DMA III -induced cell-cycle delay was still detectable.
In conclusion, the presented results reveal that the uptake capabilities of arsenic compounds are dependent upon both the cell type and the arsenic species: We noted a resistance to intracellular accumulation of arsenic, either due to increased resistance at the uptake level or an enhanced efflux mechanism by Hep G2 cells in comparison to CHO-9 cells.
The induced genotoxic effects were directly correlated to the increased intracellular arsenic concentrations. The authors thank Prof.
Obe for instructive discussions. We also thank Mrs. Gabriele Zimmer for excellent technical assistance. Conflict of interest: none declared.
Aposhian, H. Enzymatic methylation of arsenic species and other new approaches to arsenic toxicity. Occurrence of monomethylarsonous acid urine of humans exposed to inorganic arsenic.
A review of the enzymology of arsenic metabolism and a new potential role of hydrogen peroxide in the detoxication of the trivalent arsenic species.
Bau, D. Health Perspect. Basu, A. Micronuclei as biomarkers of carcinogen exposure in populations exposed to arsenic through drinking water in West Bengal, India: A comparative study in three cell types.
Cancer Epidemiol. Biomarkers Prev. Bernstam, L. Molecular aspects of arsenic stress. Health B. Bhattacharjee, H. Arsenic defence mechanisms. Broome-Smith, S.
Baumberg, C. Sterling, and F. Ward, Eds. Society for General Microbiology, Leeds. Bun-ya, M. Challenger, F.
Biological methylation. Chemical Reviews 36 , — Dopp, E. Uptake of inorganic and organic derivatives of arsenic associated with induced cytotoxic and genotoxic effects in Chinese hamster ovary CHO cells.
Interindividual variation in the metabolism of arsenic in cultured primary human hepatocytes. Gebel, T. Low-level self tolerance to arsenite in human Hep G2 cells is associated with a depressed induction of micronuclei.
Goering, P. The enigma of arsenic carcinogenesis: Role of metabolism. Ghosh, M. Gong, Z. Unstable trivalent arsonic metabolites, monomethylarsonous acid and dimethylarsinous acid.
Atom Spectrom 16 , — Gradecka, D. Selected mechanisms of genotoxic effects of inorganic arsenic compounds.
Health 14 , — Gurr, J. Calcium-dependent nitric oxide production is involved in arsenite-induced micronuclei.
Nitric oxide production by arsenite. Hartwig, A. Carcinogenesis 18 , — Modulation of DNA repair processes by arsenic and selenium compounds.
Toxicology , — Hei, T. Mutagenicity of arsenic in mammalian cells: Role of reactive oxygen species. Huang, R.
Sodium arsenite induces chromosome endoreduplication and inhibits protein phosphatase activity in human fibroblasts.
Ji, G. Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus plasmid pI Kala, S.
Kato, K. Active arsenic species produced by GSH-dependent reduction of dimethylarsinic acid cause micronuclei formation in peripheral reticulocytes of mice.
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